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  • Harvesting the genomic promise: recombineering sequences for phenotypes.

    abstract::The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-120026481

    authors: Rogatcheva MB,Rund LA,Beever JE,Schook LB

    更新日期:2003-11-01 00:00:00

  • Candidate gene approach: potentional association of caspase-1, inhibitor of apoptosis protein-1, and prosaposin gene polymorphisms with response to Salmonella enteritidis challenge or vaccination in young chicks.

    abstract::Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-120022136

    authors: Liu W,Lamont SJ

    更新日期:2003-05-01 00:00:00

  • A 3800 gene microarray for cattle functional genomics: comparison of gene expression in spleen, placenta, and brain.

    abstract::A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies. The array elements were selected from > 7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries. Sequence simil...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-120005779

    authors: Band MR,Olmstead C,Everts RE,Liu ZL,Lewin HA

    更新日期:2002-05-01 00:00:00

  • Screening and scanning of single nucleotide polymorphisms in the pig melanocortin 1 receptor gene (MC1R) by pyrosequencing.

    abstract::The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin 1 receptor gene (MC...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/ABIO-100108341

    authors: Gustafsson AC,Kijas JM,Alderborn A,Uhlén M,Andersson L,Lundeberg J

    更新日期:2001-11-01 00:00:00

  • Assessment of the nucleotide sequence variability in the bovine T-cell receptor alpha delta joining gene region.

    abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1081/abio-100102977

    authors: Fries R,Ewald D,Thaller G,Buitkamp J

    更新日期:2001-05-01 00:00:00

  • Isolation of thirty-one new porcine microsatellites from a microsatellite enriched microdissected chromosome 8 library.

    abstract::Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495390009525945

    authors: Wang Z,Rohrer G,Stone R,Troyer D

    更新日期:2000-01-01 00:00:00

  • SSCP analysis at the bovine CSN3 locus discriminates six alleles corresponding to known protein variants (A, B, C, E, F, G) and three new DNA polymorphisms (H, I, A1).

    abstract::A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H a...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495399909525921

    authors: Prinzenberg EM,Krause I,Erhardt G

    更新日期:1999-01-01 00:00:00

  • Analysis of allele segregation distortion in a swine resource family.

    abstract::Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495399909525939

    authors: Yasue H,Mikawa S,Uenishi H,Wada Y

    更新日期:1999-01-01 00:00:00

  • Identification of sequence tagged sites in the Asian and African elephant.

    abstract::To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495399809525902

    authors: Burk NE,Messer LA,Ernst CW,Rothschild MF

    更新日期:1998-01-01 00:00:00

  • Partial cloning and expression of the bovine leptin gene.

    abstract::The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...

    journal_title:Animal biotechnology

    pub_type: 杂志文章

    doi:10.1080/10495399809525887

    authors: Ji S,Willis GM,Scott RR,Spurlock ME

    更新日期:1998-01-01 00:00:00

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