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abstract::The past decade has witnessed the construction of linkage and physical maps defining quantitative trait loci (QTL) in various domesticated species. Targeted chromosomal regions are being further characterized through the construction of bacterial artificial chromosome (BAC) contigs in order to isolate and characterize...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120026481
更新日期:2003-11-01 00:00:00
abstract::Salmonella enteritidis (SE) contamination of poultry products is a major cause of foodborne disease worldwide. Caspase-1 and inhibitor of apoptosis protein-1 (IAP-1) were selected as candidate genes for chicken response to SE because their proteins play critical roles in the apoptotic pathway when intracellular bacter...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120022136
更新日期:2003-05-01 00:00:00
abstract::A cDNA microarray representing approximately 3800 cattle genes was created for functional genomic studies. The array elements were selected from > 7000 cDNA clones identified in a large-scale expressed sequence tag (EST) project that utilized spleen and normalized and subtracted placenta cDNA libraries. Sequence simil...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-120005779
更新日期:2002-05-01 00:00:00
abstract::The increasing interest in the discovery and characterization of single nucleotide polymorphisms (SNPs) emphasis the need for high-throughput and cost effective scoring methods. Pyrosequencing is a novel method for screening SNPs. In this study we examine breed specific SNPs in the pig melanocortin 1 receptor gene (MC...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/ABIO-100108341
更新日期:2001-11-01 00:00:00
abstract::The sequence of 2,193 nucleotides from the bovine T-cell receptor alpha/delta joining gene region (TCRADJ) was determined and compared with the corresponding human and murine sequences. The identity was 75.3% for the comparison of the Bos taurus vs. the Homo sapiens sequence and 63.8% for the Bos taurus vs. the Mus mu...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1081/abio-100102977
更新日期:2001-05-01 00:00:00
abstract::Chromosome 8 (SSC8) is an important one in the swine genome because it has been shown to harbor several economically important quantitative trait loci (QTL). The entire porcine chromosome 8 was microdissected and amplified by degenerate oligonucleotide primer (DOP) PCR. The PCR product was then enriched for (CA)n micr...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495390009525945
更新日期:2000-01-01 00:00:00
abstract::A high resolution SSCP protocol was developed for simultaneous discrimination of the known CSN3 alleles A, B, C, E, F and G. Furthermore, three new DNA polymorphisms were identified in different Bos taurus and Bos indicus breeds or crosses. Mendelian segregation was shown for two of these polymorphisms (named CSN3*H a...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525921
更新日期:1999-01-01 00:00:00
abstract::Segregation distortion of alleles was found in several regions of the genome in allele type analyses of genetic markers for a swine resource family that had been constructed by crossing Göttingen miniature pig and Meishan breeds. From these regions, a region on chromosome 6 presented a distortion over several consecut...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399909525939
更新日期:1999-01-01 00:00:00
abstract::To date, gene identification in elephants has essentially related to evolutionary studies. Further identification of genes in elephants could provide additional information for evolutionary studies and for evaluating genetic diversity in existing elephant populations. The objective of this project was to identify sequ...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399809525902
更新日期:1998-01-01 00:00:00
abstract::The product of the leptin (i.e., obese) gene may be an important regulator of energy metabolism, adiposity, and reproduction, and is perhaps linked to meat quality determinants such as marbling. Molecular probes were developed using polymerase chain reaction (PCR) technology to evaluate leptin expression in adipose de...
journal_title:Animal biotechnology
pub_type: 杂志文章
doi:10.1080/10495399809525887
更新日期:1998-01-01 00:00:00